VALIDATION OF A HPTLC- DENSITOMETRY METHOD FOR THE QUANTITATIVE DETERMINATION OF ANXIOLYTIC CONSTITUENTS IN STELLARIA MEDIA LINN.

Abstract

A quantitative TLC-densitometry method was developed for the determination of anxiolytic constituents of the plant — Stellaria media Linn. (Caryophyllaceae) traditionally used for the treatment of mental tension and inflammations of the digestive, renal, respiratory and reproductive tracts.

Objective: To determine anxiolytic constituents of Stellaria media Linn. by quantitative TLC-densitometry.

Methods: The separation was achieved on precoated TLC silica gel 60F254 using toluene-chloroform-methanol (6.8:2.2:1, v/v/v) as mobile phase. The plates were developed vertically up to a distance of 80 mm. TLC-densitometry method was successfully validated for linearity, precision, accuracy, robustness, limit of detection (LOD) and quantitation (LOQ).

Results: The investigation carried out revealed that contents of the two bioactive markers A1 (6-methylheptyl-3′- hydroxy-2′-methylpropanoate) and A2 (2,2,4-trimethyloctan-3-one) in the plant, were found to be 0.180 and 0.078 % w/w respectively.

Conclusion: The proposed method was found to be simple, precise, specific, sensitive, and accurate.