RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR SIMULTANEOUS DETERMINATION OF METFORMIN AND CANAGLIFLOZOIN

Mohan Goud V; Linga Kumara Swamy; Sai Prasanna D; Sandhya Rani M

Authors

Mohan Goud V Department of Pharmaceutical Analysis, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist., Hyderabad, Telangana, India.
Linga Kumara Swamy Department of Pharmaceutical Analysis, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist., Hyderabad, Telangana, India.
Sai Prasanna D Department of Pharmaceutical Analysis, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist., Hyderabad, Telangana, India.
Sandhya Rani M Department of Pharmaceutical Analysis, Joginpally B.R Pharmacy College, Yenkapally, Moinabad, R.R. Dist., Hyderabad, Telangana, India.

Keywords:

Acetonitrile, Phosphate buffer, RP-HPLC, MFM and CGF

Abstract

A simple, Accurate, precise method was developed for the simultaneous estimation of the Metformin(MFM) and Canagliflozoin (CGF) in pharmaceutical dosage form.Chromatogram was run through Kromosil 250 x 4.6 mm, 5m column. Mobile phase containingBuffer and Acetonitrile in the ratio of 65:35 with gradient method was pumped throughcolumn at a flow rate of 1ml/min. Buffer used in this method was OPA. Temperature wasmaintained at 30°C. Optimized wavelength was 220nm. Retention time of MFM and CGF werefound to be 2.424 min. and 3.730 min, %RSD of method precision for MFM and CGF were andfound to be 0.47, and 0.38% respectively. % recovery was obtained as 101.60% and 100.19%for MFM and CGF respectively. LOD, LOQ values are obtained from regression equations ofMFM and CGF were 0.005, 0.049 and 0.049, 0.150 respectively. Regression equation of MFMwas y = 21120x + 631.6 (R 2=0.999) and CGF was y = 9125x + 322.7 (R 2=0.999) foundedrespectively. The high percentage of recovery and low percentage coefficient of varianceconfirm the suitability of the method for routine estimation in pharmaceutical dosage form.

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