Development and validation of Abrocitinib In RP- HPLC

Chaitanya Kotla.S.M; Jagadhathri V; Harshitha P; Sindhuja G; Sumathi B

Authors

Chaitanya Kotla.S.M Associate Professor, Department of Pharmaceutical analysis, A.K.R.G.College of Pharmacy, Affiliated to JNTUK, Nallajerla, East godavari, Andhra Pradesh, 534112
Jagadhathri V Department of Pharmaceutical analysis, A.K.R.G.College of Pharmacy, Affiliated to JNTUK, Nallajerla, East godavari, Andhra Pradesh, 534112
Harshitha P Department of Pharmaceutical analysis, A.K.R.G.College of Pharmacy, Affiliated to JNTUK, Nallajerla, East godavari, Andhra Pradesh, 534112
Sindhuja G Department of Pharmaceutical analysis, A.K.R.G.College of Pharmacy, Affiliated to JNTUK, Nallajerla, East godavari, Andhra Pradesh, 534112
Sumathi B Department of Pharmaceutical analysis, A.K.R.G.College of Pharmacy, Affiliated to JNTUK, Nallajerla, East godavari, Andhra Pradesh, 534112

Keywords:

Abrocitinib, RP-HPLC

Abstract

A new selective reliable analytical RP-HPLC method for estimating Abrocitinib in bulk and marketed dosage form was developed and validated. The chromatographic analysis was carried on a reverse phase analytical column of Inertsil C-18 (250mm×4.6mm,5µm) column with a mobile phase of phosphate buffer PH 4.8:Methanol(55:45% v/v) at a flow rate of 1ml per min. At a wavelength of 282nm. The desired analyte was identified without any interference. The retention time of the drug was discovered to be 4.8min. The graphical method produced linear response with regression co-effiecient of 0.99 for the concentration range of 10-130µgm/ml. The LOD and LOQ values were 1.3 and 3.9µgm/ml, respectively. These results obtained, showed a good agreement with the declared content that of the formulation. Therefore , the propposed method is rapid,accurate and validated for the quantifcation of Abrocitinib in solid dosage forms.

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